First, use a wax pencil to attract a circle on the microscope slide to separate each kind of bacteria that is going to be sampled.

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If a straightforward stain of only one type of bacteria, or single blended sample of bacteria is being ready, no separation is forced.
Blog post Summary: In order to see individual bacteria through a light microscopic lense, a bacterial smear should be attached to a slide and then stained. Here is the procedure.
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In order to warm settle a bacterial smear, it is important to first let the bacterial sample air dry. Then either area the slide in the slide holder of a microincinerator, or pass the dried slide through the flame of a Bunsen burner 3 or 4 times, smear side facing up. Once the slide is warmth addressed, it can then be stained.
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In order to have the ability to clearly view individual bacteria, a sample of a bacterial colony must be combined right into water or physiological saline. This helps to evenly spread out the bacterial sample.

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Bacterial smear after heat addressing and prior to staining. Positive bacterial control in circle on the left, negative regulate on ideal, and unrecognized bacteria in center circle. 
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